In Vitro Services

The in vitro services at Toxikon offer a full range of study with applications to assist customers in the drug development process, drug discovery, IND, Phase I-III trials, and NDA submissions. The in vitro assays provide important information, including metabolism, cytotoxicity, for investigational candidates that is essential for pharmaceutical product development. All studies are conducted in compliance with GLP regulations.

Quality Assurance Unit monitors all aspects of drug metabolism studies, from the development of study specific SOPs, through the conduct of the study, to report, preparation, and finalization.

In Vitro Services Include:

• Cell Viability (Cytotoxicity)
• Cell Proliferation Assay
• Study the species variation in metabolism using cells, both hepatic (e.g. hepatocytes) and extrahepatic (e.g. keratinocytes), and subcellular fractions, such as microsomal and S9 preparations
• Study drug-drug interactions that may occur due to Cytochrome P450 (CYP450) inhibition or induction
• Study induction of CYP450 by compounds using CYP-specific assays and Western immunoblotting
• Metabolite stability, profile, and identification
• Enzyme and immunoassays such as ELISA, RIA

All the in vitro assays can be performed using compounds/test articles that are both radiolabeled and non-radiolabeled.